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Subcutaneous injection technique involves cleaning the injection site with alcohol, pinching skin to create a fold, inserting the needle at a 45-90 degree angle, and injecting slowly

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But long-term data concerning the course of micronutrient status is still missing

In all our previous murine studies we have used short cycles (1014 days) of very low subcutaneal doses (20 g/kg/day) after carefully assessing its circulating concentrations and using the minimum amount that was sufficient to adequately restore physiological values of the molecule

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67 , e2200450 (2023)