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previous blogs entitled as: Developmental Delays in Infancy and Early Childhood: Metabolism of Homocysteine

In a Burkitts lymphoma model, toxicity to ascorbate was associated with ROS-induced peroxide formation that could be abrogated by catalase treatment ( in vivo in the microenvironment of three separate models where vitamin C reduced tumor burden in mice ( The pro-oxidant effects of vitamin C in these studies has been attributed to excess uptake and formation of redox active iron

HeLa cells assay AtSNX1 and MmSNX1 CDS were cloned into the plasmid pLV-IRES-EGFP to generate GFP-tagged forms and then infected HeLa cells with lentiviruses packaged using the pLV-IRES-AtSNX1-EGFP and pLV-IRES-MmSNX1-EGFP plasmids, respectively, to obtain HeLa-AtSNX1-GFP and HeLa-MmSNX1-GFP cell lines