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MDA-T32 cells exhibited RSL3-induced, disproportionately robust rise in intracellular ROS levels in concentration-dependent manner when compared to control HThF cells

Similarly, in the Mfn1 and Mfn2 double-knockout mouse model, spermatocytes showed mitochondrial fragmentation and reduced mitochondrial content, abnormal cristae, and lower numbers and activity of OXPHOS subunits (NdufB6 and MTCOI), resulting in obstruction from the leptotene to the pachytene stage, inhibiting spermatogenesis, and leading to a significant decrease in sperm [46]

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