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Functional enrichment analysis indicated these intersecting genes are primarily involved in biological processes including oxidative stress responses, smooth muscle cell proliferation, and reactive oxygen species (ROS) responses, closely matching RA pathological features such as excessive inflammatory factor release and oxidative stress injury ( We further applied three classical machine learning algorithms (LASSO, RFE-RF, and SVM) to cross-validate and identify two core genes, GPX3 and MYC, which were subsequently validated using an MLP model

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Proteasome inhibition prevents experimentally-induced endothelial dysfunction

Western blot HEK-293T cells expressing hCC WT or the L68Q variant were washed twice with ice-cold phosphate-buffered saline (PBS) and lysed on ice using a freshly prepared ice-cold cell lysis buffer containing 50 mM Tris-HCl, pH 7.4, 100 mM NaCl, 50 mM -glycerophosphate, 10% glycerol (w/v), 1% NP-40 (w/v), 1 mM EDTA, 2 mM NaVO4, and a complete, EDTA-free, protein inhibitor cocktail (Roche Applied Science, Mannheim Germany) at 20 l per mL of lysis buffer

M.YakubuM

Expression of growth hormone and growth hormone receptor genes in human eye tissues