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Following normalization using the housekeeping gene, the 2Ct technique was used to calculate the fold changes in the expression levels, as described in 2.7 Detection of aflatoxin residues in eggs The egg was collected after the completion of the trial to detect the residual AFT using high-performance liquid chromatography (HPLC)

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The TGR sample (1/50 volume cell culture) was applied to a 2.5 ml column, which was then washed with 50 ml of TE after which TGR was eluted in ten 1 ml fractions of 1 mM NADPH in TE

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However, though some studies also reported some plasma biomarkers for brain oxidative damage by analyzing different kinds of samples including F4-Neuroprostanes and F2-Dihomo-Isoprostanes (278), biomarkers in peripheral body fluid samples are still insufficient to identify brain or peripheral oxidative stress at the moment