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4 Apparently, the neutrophils from fibromyalgia patients have undergone changes that can activate the ganglia and ramp up pain

For intracellular cytokine staining, cells were incubated with Brefeldin A (1:1000, #00-4506-51, Invitrogen, USA), Monensin (1:1000, #00-4505-51, Invitrogen, USA), PMA (10 ng/mL, #P8139, Sigma, USA) and ionomycin (500 ng/mL, #FMS-FZ208, Fcmacs, Nanjing, China) at 37 C for 3.5 h

Visible results are possible in a single session, but you can achieve better results with multiple sessions

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