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Grape polyphenols prevent fructose-induced oxidative stress and insulin resistance in first-degree relatives of type 2 diabetic patients

Cells were seeded on collagen-coated plates (250 g/ml rat collagen, Roche) at a density of 150,000 cells/cm 2 and cultivated for 3 h in Williams E medium (PAN Biotech) containing penicillin (100 U/ml, Gibco), streptomycin (0.1 mg/ml, Gibco), gentamycin (10 M, PAN Biotech), dexamethasone (100 nM, Sigma), stable l-glutamine (2 mM, Sigma), insulin supplement (2 ng/ml, Sigma), and 10% Sera Plus (PAN Biotech)

10.1038/nchembio.2239 63 DoshiB

483,484 The peptide p5RHH has been efficiently used to transfect siRNA and mRNA into human cartilage to reduce chondrocyte apoptosis and prevent cartilage degeneration 485,486,487 as well as into cardiac tissue for treating abdominal aortic aneurysm to reduce the risk of aortic rupture and sudden death in mice

Further research is imperative to fully understand the potential roles and mechanisms of these pharmaceutical agents pertaining to the selective targeting of ferroptosis within the context of MASLD