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Injection into sensitive or low-fat tissue: Areas such as the abdomen, inner thigh, or other sites with minimal subcutaneous fat have less tissue cushioning and a higher density of sensory nerve endings, making pH-related discomfort more noticeable

doi: 10.1007/s00726-007-0471-2

These plasmids were constructed by the Gibson assembly method using DNA fragments amplified through PCR with the primer sets listed in Supplementary Table 2