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After 48 h the cells were washed then fixed and permeabilized with cold 70% ethanol for 2 h at -20C then washed with cold PBS twice

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28 However, owing to the lack of advanced technologies at the time, the critical role of nucleic acids was not fully understood

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Consequently, by inhibiting the cascade amplification of peripheral inflammatory signals to the central nervous system, it indirectly alleviated central nervous system inflammation and exerted a neuroprotective effect ( 4 Conclusions and outlook Treatment for PD must address both motor and non-motor symptoms