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Therefore, targeting the dysfunctional mitochondria accompanying ROS imbalance and regulating antioxidant enzymes are potential targets to enhance bone health

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The day of the experiment, cells were treated with test compounds for the indicated times, then harvested by trypsinization, resuspended in 500 L Hanks Balanced Salt Solution (HBSS, Gibco, Invitrogen Corp., Carlsbad, Calif.) containing either H 2 DCFDA (25 M), C11-BODIPY(581/591) (2 M) or MitoSOX (5 M) (all from Molecular Probes, Invitrogen) and incubated for 10 minutes at 37 C

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You will use two boxes of Novarel 5,000 IU for your trigger injection

Bo G, Bo-Yu L, Shou-Liang Y, Yue-Hua L, Guang-Yue L (2021) A time-dependent density functional theory study of a fluorescent probe to detect hydroxyl radicals: Inhibiting the twisted intramolecular charge-transfer process