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Balaban M, Jiang Y, Zhu Q, McDonald D, Knight R, Mirarab S

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(B) Images captured from the same experiment as A (with no Alexa Fluor 488 secondary to detect total GSTP1) (i) in the absence of -alkynyl-palmitate and (ii) with no click chemistry step

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Cells are then permeabilized in 0.2% Triton X-100/PBS for 5 min, and blocked with 10% normal goat serum (Jackson Immuno Research Labs, West Grove, PA) for 1 h