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Glutamate and neurogenesis (player behind the curtain) Neurogenesis is the ability to build not only more neurons but more connections between them

Afterwards, cells were washed with PBS, and incubated at room temperature for 30 min with an anti-rabbit IgG Alexa Fluor 546 antibody (1:100, #A-11035, Thermo Fisher Scientific, Waltham, MA, USA)

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Quantitative real-time PCR (qRT-PCR) was performed using SYBR Green PCR master mix (Roche Applied Science, Mannheim, Germany)

Rhodamine dyes have photophysical properties such as high fluorescence quantum yield, high molar extinction coefficient and good water solubility, and low biological toxicity, making them attractive for wide use as biomarkers and fluorescent probes