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In addition, butylmalonate, an inhibitor of DIC, significantly decreased mGSH, suggesting DIC as the major GSH transporter in mouse cerebral cortical mitochondria (Kamga et al., 2010)

and which of the Cys was more likely to be modified

The brain has several mechanisms to neutralize ROS, of which antioxidant scavenging is one of the final lines of defense [6]

Several candidate non-glutathione disulfide substrates for the dithiol mechanism have been identified using monothiol glutaredoxin mutants as bait proteins for the trapping of Grx(SSR) species (Fig

Cells were incubated for 10 min at room temperature and then 20 L of 10% CHAPS detergent (Sigma-Aldrich, C3023) was added, and cells incubated at room temperature for an additional 10 min to lyse cells

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