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The process of macroautophagy (herein referred to as autophagy) can be divided into 5 stages: initiation, elongation, maturation, fusion and degradation [8]

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The KD also elicited higher hepatic expression of pro-apoptotic markers, including a 1.8-fold increase in cleaved caspase-3 compared to high-sugar diets and a 2.1-fold elevation in the tumor suppressor protein p21

Metformin treatment (100 mg/kg) showed the most significant protective effect, with a marked reduction in lipid accumulation, improved cellular integrity, and minimal evidence of necrosis or other pathological features ( E )

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However, the subcutaneous route of administration is often preferred for administration of research peptides