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In vitro BP incorporation was performed at 37 C for 1 h, and then, the samples were diluted with sample loading buffer (60 mM Tris-Cl (pH 6.8), 10% glycerol, 2% SDS, 1% -mercaptoethanol (ME), 0.01% bromophenol blue) and boiled at 100 C for 10 min

19 The clip was applied for 5 min with approximately 1.5 N of holding force (Figure 1Ai)

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In particular, the importance of an adequate consumption of trace elements such as selenium (Se), zinc, copper and iron, is supported by their effects on the activation of the immune system for the protection of infections caused by bacteria, viruses and parasites

DCB, drug-coated balloon