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Our findings, combined with the prior report from Qiu et al

Weigand T, Colbatzky F, Pfeffer T et al (2020) A global Cndp1-knock-out selectively increases renal carnosine and anserine concentrations in an age- and gender-specific manner in mice

RhIGF-1 resulted in significant, sustained increases in GFR (4281%) over a treatment period of 45 days

As concern the first, the cathepsin-mediated cell death, our data are in line with those provided by other authors, demonstrating that inhibitors of autophagy, i.e., CQ and the phytochemical thymoquinone, could mediate lysosome permeability, resulting in leakage of cathepsin B into the cytosol where it can act as mediator of cell death 47

While the 500 mol NMDA concentration in the blood causes ROS increase compares to the control, a lower dose of 50 mol NMDA cause more than three times higher ROS production [33]