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For homogenization of the tissue samples, we added trichloroacetic acid (TCA)

J Med Chem 37 : 15011507

J Allergy Clin Immunol 118:455465

Zhou X, Liu H, Zhang J, Mu J, Zalan Z, Hegyi F, Takacs K, Zhao X, Du M (2019) Protective effect of Lactobacillus fermentum CQPC04 on dextran sulfate sodium-induced colitis in mice is associated with modulation of the nuclear factor-kappaB signaling pathway

Following this, the protein lysates were denatured in a metal bath at 95 C and subjected to SDS-polyacrylamide gel electrophoresis (SDS-PAGE) for separation