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These complexes use synthetic guide RNA to direct a double-stranded DNA break

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For CH, its bands at 3504.65 cm 1 , 1660.66 cm 1 , 1407.99 cm 1 and 1083.97 cm 1 related to axial stretching of O-H group, which appears superimposed to N-H stretching, C = O stretching of acetyl units, N-H group and C-O and C-O-C stretching, respectively

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A mixture of buffer and NaN 3 solution was prepared at a volume ratio of 17:1

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