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In addition to the incubation of nanozymes with H 2 O 2 at a clinically relevant concentration, we also employed the hydroxyl radical and SOD detection kits to profile the hydroxyl radical scavenging behavior and SOD-like activity of nanozymes under physiological and pathological microenvironments over 28 days

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In particular, we demonstrated that N-linked glycosylation regulates the stability and secretion of proIGF-1Ea, probably ensuring proper prohormone folding and favouring its passage through the secretory pathway

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However, many of these studies involved co-supplementation with other nutrients such as magnesium, calcium, and vitamin D, making it difficult to isolate the specific effects of zinc alone