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Electroporation After a 1-week acclimatization, cells were collected at a density of 1 10 6 cells and mixed with plasmid diluted in Opti-MEM reduced serum medium (Thermo Fisher Scientific)

After discarding the supernatant, islets were incubated in KRBH containing 0.1% BSA, 1 mM glucose and indicated concentrations of somatostatin (SST-14) or insulin at 37 C for 1 h

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