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Reference Shishido, Tomoike, Kuwata, Fujikawa, Sekido, Murakami-Tonami, Kameda, Abe, Kimura, Shuto and Abe2019)

Funding: This research was supported by National Institutes of Health (NIH) R21: NIH R21 NS075500 to JMG and SNR, and in part by NIH R21AG038989 grant to JMG

Invited Review: redox modulation of skeletal muscle contraction: what we know and what we dont

The mixtures for the SPS analysis contained 50-mM TrisHCl (pH 7.0), 10-mM MgCl 2 , 10-mM fructose-6-phosphate, 3-mM UDP-glucose, and 100 L of crude enzyme

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This suggests that the test extract, regardless of concentration, did not drastically alter the acidity or alkalinity of the medium