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To test this prediction, we mutated VadK-His422 to alanine, checked that VadK His422Ala was folded in a similar manner to VadK WT by circular dichroism (CD) (Appendix Fig

Treatment-emergent antibodies specific to amustaline/GSH RBCs were observed with a predicate pathogen reduction device [8] and could be neutralized with acridine in solution, thereby defining specificity for the acridine moiety of the amustaline molecule (also known as S-303)