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First, the above four cell lines were inoculated in a confocal culture dish at a density of 1 10 5 per well and cultured in incubators (37, 5% CO 2 ) for 24 h

doi: 10.1371/journal.pone.0023211 [DOI] [PMC free article] [PubMed] [Google Scholar] 73

McGoldrick, S., OSullivan, S

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79,80 In mammalian cells, the GATOR1 and GATOR2 complexes exhibit weak direct interactions and rely on the tetrameric KICSTOR complex and the KICSTOR-interacting VWCE protein to anchor GATOR1 to lysosomes under conditions of amino acid or glucose deprivation