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Zhang et al.308 analysed the expression of certain virulence factors in vivo and in vitro concluding that in vitro measurements of transcription compared to transcription in infected lung tissue demonstrated low levels of fos-1 and rhbA genes, and 2040-fold increases in cpcA, lysF, and pabA genes, while the pksP gene was only detected in vivo

Ubiquitin constructs used include WT (wild-type), K48R, K63R, K48 (all ubiquitin lysines mutated to arginine except K48) and K63 (all ubiquitin lysines mutated to arginine except K63)