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After 3D classification, two types of inward-facing conformations with variable distance between the two NBDs were identified (the different opening angles for different classes are visualized in Supplementary Fig

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However, membrane proteins with ectodomains larger than a single IgG domain, which constitute approximately 51% of human surface proteome (2) (see Materials and Methods), have heights that are not simply the sum of their individual domains due to the domain organization, flexibility between domains, and thermally-driven fluctuations that reduce average extension from the surface

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