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Samples were extracted for thioacidolysis as described above and the percentage of label incorporated into the lignin monomers was calculated as follows: % 13 C incorporated = peak area labeled/(peak area labeled + peak area unlabeled) 100

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Centrifuge at 1,500g for 10 minutes at 28C

This framework thereby bridges molecular mechanisms to clinical disease, demonstrating oxidative stress as a unifying axis in multifactorial pathologies

Enhanced gastrointestinal passive paracellular permeability contributes to the obesity-associated hyperoxaluria