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Complete RPMI phenol red-free media (Gibco) was used and was beforehand completed with CellTox Green Dye at a 1:500 ratio (v/v)

AHK-Cu stimulates fibroblast proliferation and activity, leading to increased protein deposition in the extracellular matrix

The mechanisms include chronic low-grade inflammation, an altered gut microbiota, and often a calorie-dense but nutrient-poor diet

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None of the patients or controls had received B 12 or folate supplementation within the preceding year