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After a wash step, cells were incubated with fixation/permeabilization concentrate for 30 minutes to fix the surface and permeabilize cells for intracellular staining

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Ethical principles of the study ensured compliance with European Directive 010/63/E, the Law on Amendments to the Animal Protection Act (Official Gazette 37/13, the Animal Protection Act (official Gazette 135/06), Ordinance on the protection of animals used for scientific purposes (Official Gazette 55/13), FELASA recommendations, and recommendations of the Ethics Committee, School of Medicine, University of Zagreb

Essential vitamins for sustaining the heart, neurological system, and digestive system include vitamin B1 (thiamine), vitamin B2 (riboflavin), vitamin B3 (niacin, nicotinic acid), vitamin B5 (pantothenic acid), and vitamin B6 (pyridoxine and derivatives)

Because of this, the effects in human patients are unconfirmed

Elevates NAD+ and S-adenosylmethionine (SAM) levels, shifting metabolic flux toward energy expenditure and away from fat storage 1-4