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Altogether, using multiple screens, independent methods and extending the Hits by an ortholog search, we have identified a list of high confidence S-palmitoylated protein hits, which could be useful for further study

In cultured fibroblasts, GHK-Cu increased collagen synthesis at strikingly low concentrations the effect began between roughly 10 -12 and 10 -11 M and peaked near 10 -9 M, independent of any change in cell number, meaning the peptide upregulated matrix production per cell rather than simply growing more cells

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Possible solution: Cross-link the GST fusion protein to the GSH beads, or reduce the amount of GST fusion protein used

C57Bl/6 N mice were ordered from Janvier Labs, and an acclimatization period of 3 weeks was included before any experiment

In: Thoracic Surgery, 637-647, 1993