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After 24 h of LPS treatment, the medium was harvested and luciferase activity was measured using the Secrete-Pair Dual Luminescence Assay Kit (GeneCopoeia)

This work was supported by the National Key Research and Development Program of China (2022YFA1104300 to Hongbo Xin and Keyu Deng), the National Natural Science Foundation of China (82470454 and 82270302 to Hongbo Xin, 81970256 to Keyu Deng and 82560089 to Lingfang Wang), the Natural Science Foundation of Jiangxi Province, China (2025BAC240701 to Lingfang Wang), and the Jiangxi Province Key Laboratory of Bioengineering Drugs (No

3) 12-week-old male Sprague-Dawley rats were assigned to different treatment groups and used for animal experimentation in accordance with NIH guidelines

CypA, Cyclophilin A

Glutathione peroxidase 4 (GPX4) is a key enzyme in iron dependent death that can reduce the production of lipid hydroperoxides (PE-AA-OH and PE-AdA-OH)

8A and 8B )