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At a more complex level, the tear film is composed of electrolytes (e.g., sodium, chloride), antioxidants (e.g., vitamin C, glutathione), anti-infectives (e.g., lactoferrin, lysozyme), immune and inflammatory proteins (e.g., sIgA, IgG, IL-2), proteins involved in tear-film maintenance (e.g., lipocalins, mucins), proteins involved in corneal wound healing and maintenance (e.g., fibronectin, TGF-alpha, insulin), neuropeptides (e.g., calcitonin gene-related peptide, subP) and lipid components (e.g., wax esters, cholesterol esters).2-4 These tear-film components originate from a variety of sources, including the lacrimal glands, ocular surface cells, nerve endings and meibomian glands

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