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Y., Chopp, M., Zhang, Z

The fungus penetrates the layers of the cuticle through a combination of mechanical pressure and enzyme degradation (Zaki et al., 2020)

During sperm maturation, most histones are replaced by protamines to tightly package DNA and protect genetic information [187]

2,6-Dichlorophenolindophenol (DCPIP) was purchased from Merck KGaA (Darmstadt, Germany)

*Correspondence: Bin Yang, [email protected] Zhang, [email protected] Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

2.7 Determination of SOD, CAT and glutathione peroxidase (GSH-P X ) concentration After thawing, 200 L of semen was transferred to a 1.5 mL centrifuge tube and centrifuged at 1600 g for 10 min at 4 C