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Luciferin is oxidized to oxyluciferin by the Ultra-Glo TM rLuciferase, leading to emission of chemiluminescence, which was measured using an integration time of 1 s/well by the CLARIOstar microplate reader (BMG Labtech)

The area of the rhizosphere is predicted to be an area of increased acidic stress, as throughout their life cycle, plants can excrete acidic compounds into the surrounding soil, decreasing the pH of the soil by as much as 2 pH units (Faget et al., 2013)

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This only means that a solution may appear clear, yet chemical changes have already occurred

Clinical translation pathways The clinical translation of amino acid metabolic modulation requires establishing a multi-tiered "biomarker validation-intelligent decision-making-precision intervention" pathway, encompassing multicenter validation of metabolic biomarkers and AI-powered therapeutic modeling integrating multi-omics data