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found that choline supplementation increases global DNA methylation and the expression of peroxisomal acyl-coenzyme A oxidase 1, which mediates FA -oxidation

To generate Rac1-pGEX-6P-1 for bacterial expression, the pGEX-6P-1 vector was digested with BamHI and NotI, the human Rac1 sequence was PCR-amplified from pRK5-myc-Rac1-WT and inserted using Gibson assembly