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(1979) and reported as thiobarbituric acid substance (TBARS) level

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doi: 10.1016/0304-3940(89)90513-2

This suggests that NAPstar4.3 is in an almost fully NADP + -bound state in the yeast cytosol, unable of responding to a further oxidation of the NADP redox couple ( Figure S6 )

After cleavage with His-TEV to remove the His-MBP tag, the eluted fraction (SNX1-His-SUMO-SNX2a) was further loaded onto a Ni-NTA column and washed with buffer containing 20 mM HEPES (pH 7.4), 200 mM NaCl, and 40 mM imidazole to remove unbound samples