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Analysis of a conditional degradation signal in yeast and mammalian cells

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Clonal isolates of vectors bearing recombinant segments are used to infect separate cultures of cells and the percentage of vectors which enter cells is then determined by, for example, counting cells expressing a marker expressed by the vectors in the course of transfection The resulting library of recombinant transfer modules are screened to identify at least one optimized recombinant transfer module that enhances the capability of a vector comprising the transfer module to enter a cell of interest