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EOD dosing appears sufficient for anti-aging goals where the downstream gene expression changes outlast the peptide's plasma clearance GHK-Cu's impact on collagen and elastin gene expression persists for days after a single dose, meaning you don't necessarily need daily systemic presence

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Finally, the DNA was resuspended in endotoxin/DNase-free water and its concentration measured using a NanoDrop UV-Vis Spectrophotometer (Thermo Fisher Scientific), with the purified DNA stored at -20C

Morphologically, the finger-like muscle cell endings were preserved (Figure 12, dashed border)