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HEK293F cells were typically infected with a 7% (v/v) volume of infection of the P2 virus at a density of 2.53.0 10 6 ml 1

0.2 mL delivers the 3 mg target

A central conundrum in immunosenescence research lies in navigating the dual risks of therapeutic interventions: suppressing age-associated inflammation (inflammaging) may inadvertently exacerbate infection susceptibility or compromise basal immune homeostasis, whereas overactivating inflammatory pathways to restore immune function risks precipitates autoimmune pathologies or acute inflammatory sequelae

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