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Immunoblotting Cells were washed with PBS and lysed with lysis buffer (150 mM NaCl, 20 mM EDTA, 50 mM Tris-HCl, 0.5% NP-40) containing protease and phosphatase inhibitors

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rs6276, rs6277, and rs1800497) in the DRD2 gene are associated with decreased D 2 R expression and function attributable to decreased D 2 R messenger RNA stability and synthesis of the receptor and are associated with elevated blood pressure and hypertension [10, 39]