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Vest et al., Reference Vest, Leary, Winge and Cobine2013

Considering the role of Mn 2 + in activating the cGAS-STING pathway and generating hydroxyl radicals (OH) through Fenton-like reactions, coupled with the ability of Que to induce apoptosis, we further investigated MQ-induced immunogenic cell death (ICD)

The accumulation and impaired clearance of uremic toxins represent the core manifestation of bidirectional gut-kidney axis regulation

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Furthermore, metformin significantly induced necrosis in HCT116 cells, when compared to vehicle control ( Figure 3C )

Lentivirus production Lentivirus was produced in HEK-293T cells which were transfected using Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA, USA) with the plasmid of interest, a packaging plasmid psPAX2, a gift from Didier Trono (Addgene plasmid # 12260