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hPSCs were dissociated into single-cell suspensions using TrypLE Select (Thermo Fisher Scientific, Netherlands) and plated at a density of 2 10 6 cells/well in Stem Flex medium (Thermo Fisher Scientific, Netherlands) supplemented with 10 M Y-27632 (Peprotech, USA) on Geltrex-coated 6-well plates (Eppendorf, Germany) under a 5% CO 2 atmosphere

This is because retinol is a gold-standard anti-aging ingredient

Twice-daily dosing (500mcg morning and evening) shows up in more aggressive protocols but adds cost and injection logistics on top of an already-active TRT schedule

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however, they noted that the dose may have been too low to achieve a statistically significant benefit