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The lipidomics data generated in this study have been deposited in Metabolomics Workbench under Project ID PR002013 102

Additionally, a heat treatment (heating at 55C for 5 min) was performed with variants that were sufficiently stable, to induce precipitation of apo enzyme (Smith et al., 2023)

"What is your emergency protocol?" (Facilities administering IVs must be equipped to handle anaphylaxis)

doi: 10.1038/s41587-019-0114-2 13 BechtEGiraldoNLacroixLButtardBElarouciNPetitprezFet al

In both HES lines, Grx1-roGFP2-HES2 and mito-Grx1-roGFP2-HES2, the sensor reacted in a concentration dependent manner to oxidation by H 2 O 2 with an increase in fluorescence ratio (R/R 0 ) under 405 nm excitation over 488 nm excitation (Figure 2E,F)

In contrast, [U- 13 C]glucose isotope tracing results demonstrated a significant increase in TCA cycle intermediates derived from glucose when GGCT was overexpressed