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Always consult a licensed physician or qualified healthcare professional regarding any health-related questions, and consult appropriate regulatory guidance before purchasing, handling, or storing any research chemical or reagent

Dulbecco's modified Eagle's medium for cell culture, fetal bovine serum, and trypsin were purchased from Gibco (Grand Island, USA)

The Prevalence of Cobalamin Deficiency Among Vegetarians Assessed by Serum Vitamin B12: A Review of Literature

Excellent dietary sources include: Meat : beef, lamb, and pork (particularly liver, though liver should be limited in pregnancy due to high vitamin A content) [3] [4] Fish : salmon, sardines, and tuna (limit tuna to 2 steaks or 4 medium cans weekly due to mercury content) Poultry : chicken and turkey Dairy products : milk, cheese, and yoghurt Eggs : particularly the yolk For women following plant-based diets , obtaining sufficient B12 from food alone is challenging, as plant foods do not naturally contain bioavailable B12

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[20] [21] These changes lead to reduced appetite, earlier satiety, and metabolic improvements including enhanced insulin sensitivity