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In response to the detection of microtoxicants, human bronchial epithelial cells produce a variety of cytokines, chemokines, and other signaling molecules, including interleukins (IL-1, IL-1, IL-6, IL-8) and granulocyte-macrophage colony-stimulating factor (GM-CSF), which contribute to the activation of airway inflammation ( Figure 1) [9, 14]

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After 4 h at 37C, the reactions were treated with DNase I (Roche), extracted with phenol-chloroform, and dialyzed using 3,500-MWCO tubing (BioDesign Inc

277 Due to the lack of sufficient clinical evidence of the long-term safety of the KD, the use of KD in chronic diseases remains debatable

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2012;34(10):12817 Veringa SJE, Nanayakkara PWB, van Ittersum FJ