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The peak fractions were concentrated on a 50 kDa MWCO Amicon concentrator and applied onto a Superdex 200 10/300 column (Cytiva) pre-equilibrated in SEC buffer (20 mM HEPES pH 7.5, 300 mM NaCl, 5% glycerol, 1 mM TCEP)

This diet is a dietary pattern that limits highly refined carbohydrates, added sugars (including sugar, honey, jam), dairy products, and cured and red meats

doi: 10.1016/j.tcb.2023.07.009 73 LuS

doi: 10.1186/s12958-023-01055-z 255 Di TucciCDi FeliciantonioMVenaFCaponeCSchiaviMCPietrangeliDet al

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Effective electrical stimulation by a poly (l-lactic acid)/vitamin B2-Based piezoelectric generator promotes wound healing