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DMEM Dulbecco's modified Eagle medium Human HT-1080 fibrosarcoma cells were originally obtained from ATCC and were cultured in DMEM (Invitrogen) supplemented with MEM NEAA (Invitrogen), 10% (vol/vol) FCS, 100 U/mL penicillin, and 100 g/mL streptomycin

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They can be arranged in double layers together with intact and peripheral membrane proteins

doi: 10.1038/s41467-019-13601-8 56 WangHSunJSunHWangYLinBWuLet al

Most of the weight lifters were in different weight classes, so they were competing against themselves

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