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The super natant was diluted in the proportion of 1 to 4 volumes of plug and incubate to 37C, for one hour with agitation of different melatonin concentrations or homeopathic medicine according to the following tables: TABLE 3: Melatonin Concentrations used in the essay TABLE 4: Medicines used and their respective potencies Following, 1ml of the mixture was taken out from all incubated bottles and 1 ml of TCA 5% was added, what is equal to the MDA BASAL ( time of the reaction)

Mulder GD, Patt LM, Sanders L, Rosenstock J, Altman MI, Hanley ME, et al

Jung and Kim 2013

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sativus filtrate that acts as both the reductant toward a rapid reduction of Ag ions and the stabilizing agent responsible for protecting the formed AgNPs ( It should be emphasized that after 60 min, the SPR peak of the AgNP colloid attained an optical density of approximately 2.0 ( Supplementary Figure S1b )