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Future investigations on how the biological clock governs cell proliferation and growth and on the potential implications for the onset of human diseases are warranted

Abdalla S, Makhoul G, Duong M, Chiu RC, Cecere R

J Park Dis (2018) 8(s1):S11521

For the wash-out experiments cells were similarly handled but washed three times with Opti-MEM + 1% FBS (100 l) before adding 100 l fresh media with 6 ng/ml PMA solution without any inhibitor for 6 h (Wash-out)