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Ovarian steroids regulate gene expression in the dorsal raphe of old female macaques

Araki K, Turner AP, Shaffer VO, Gangappa S, Keller SA, Bachmann MF, et al

Sanelli, T

L.HammelH

The release of the GLP-1 receptor agonist in the distal small intestine (distal jejunum or ileum) is advantageous in view of the reduced activity of proteolytic enzymes as compared to the proximal small intestine (duodenum and jejunum), in view of the reduced intestinal motility in this segment (leading to reduced dilution effects of the dissolving pharmaceutical composition, enabling a high concentration of the GLP-1 receptor agonist to achieve optimal absorption), and in view of the higher solubility of peptidic GLP-1 receptor agonists at the pH levels present in the distal jejunum or ileum as compared to those present in the stomach, duodenum or proximal jejunum

Subsequently, the peptide solution was diluted 1:1 using 0.2% (v/v) NP-40 in PBS, and isoDTB-conjugated peptides were enriched on high-capacity streptavidin agarose beads (50 l slurry, Fisher Scientific, 10733315) in 0.1% (v/v) NP-40 in PBS for 1 h at r.t while rotating (disc rotator)