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(C) ROS level in basal condition (5% FBS) and serum-deprived HMVECs treated with PBS (vehicle, 12h), L-aminocarnitine (L-amino, 1M, 12h) and/or PLC (1mM, 12h)

At the same time, compared to HCs, the levels of a wide range of metabolites such as glutamine, asparagine, N-Formimino-glutamate, putrescine, serotonin, kynurenine, 2-Aminomuconate, Cys-Gly, 5-Aminolevulinate, and pyridoxine were reduced in the plasma of R group ( Figure 5C , Supplementary Figure 4A )

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Notably, N-acetylglutamate in metabolites may serve as an indicator of skin cell energy metabolism and subsequent adaptive physiological changes

Second, in the 1980s, vascular endothelial cells were shown to produce nitric oxide from l-arginine, which explained the biological effects attributed to endothelium-derived relaxing factor (Furchgott and Zawadzki 1980